Characterisation of the Brochothrix thermosphacta sortase A enzyme
نویسندگان
چکیده
منابع مشابه
End Products of Glucose Fermentation by Brochothrix thermosphacta.
Anaerobically, Brochothrix thermosphacta fermented glucose primarily to l-lactate, acetate, formate, and ethanol. The ratio of these end products varied with growth conditions. Both the presence of acetate and formate and a pH below about 6 increased l-lactate production from glucose. Small amounts of butane-2,3-diol were also produced when the pH of the culture was low (</=5.5) or when acetate...
متن کاملReduction of Brochothrix thermosphacta on beef surfaces following immobilization of nisin in calcium alginate gels.
Lean and adipose beef carcass tissues inoculated with Brochothrix thermosphacta (BT) (approx. 4.50 log10 cfu cm-2) were left untreated (U) or treated with 100 micrograms ml-1 nisin (N), calcium alginate (A) or 100 micrograms ml-1 nisin immobilized in a calcium alginate gel (AN). Tissue samples were refrigerated after treatments and bacterial populations and nisin activity were determined at 0, ...
متن کاملEffect of modified atmosphere composition on the metabolism of glucose by Brochothrix thermosphacta.
The influence of atmosphere composition on the metabolism of Brochothrix thermosphacta was studied by analyzing the consumption of glucose and the production of ethanol, acetic and lactic acids, acetaldehyde, and diacetyl-acetoin under atmospheres containing different combinations of carbon dioxide and oxygen. When glucose was metabolized under oxygen-free atmospheres, lactic acid was one of th...
متن کاملGrowth of Brochothrix thermosphacta in ground beef following treatments with nisin in calcium alginate gels
Sterilized, lean and adipose beef carcass tissues were inoculated with Brochothrix thermosphacta, left untreated (U), or treated with 100 μg/ml nisin (N), calcium alginate (A), or 100 μg/ml nisin immobilized in a calcium alginate gel (AN). Treated tissues were aseptically processed into ground beef and populations of B. thermosphacta and nisin activity were determined during refrigerated storag...
متن کاملReductions of Listeria innocua and Brochothrix thermosphacta on beef following nisin spray treatments and vacuum packaging
Sections of UV sterilized lean and adipose tissues from the surfaces of post-rigor (24 h post-mortem) beef carcasses were inoculated with Brochothrix thermosphacta or Listeria innocua to obtain approximately 4·50 log10 cfu cm and subjected to spray treatments with sterile water or nisin (5000 AU ml). Untreated and spray treated samples were vacuumpackaged, and incubated at 4°C for up to 4 weeks...
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ژورنال
عنوان ژورنال: FEMS Microbiology Letters
سال: 2018
ISSN: 1574-6968
DOI: 10.1093/femsle/fny184